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Image Search Results
Journal: Communications Biology
Article Title: Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors
doi: 10.1038/s42003-021-01740-y
Figure Lengend Snippet: a Representative maximum intensity projections of 100 mM EGF stimulation of HeLa cells expressing APPL1-EGFP and EEA1 TagRFP-T (magenta). Hollow arrows point at a few examples of APPL1 and EGF colocalization and the solid arrows point at APPL1-EEA-EGF1 triple or EEA1-EGF double positive endosomes. Scale bar = 15 µm. b A zoomed view of APPL1-EEA1-EGF triple positive endosomes in the boxed region in ( a ). Scale bar = 0.5 µm. c An EGF-APPL1 double positive endosome undergoing maturation by conversion to an EGF-EEA1 positive endosome. Scale bar = 0.2 µm. d Graph of fraction of EGF tracks positive for APPL1 and EEA1. Error bars indicate standard deviation ( n = 6 cells). e Same plot as ( d ) for cells treated with 50 µM ciliobrevin ( n = 5 cells). Double lines on the x -axis indicate breaks in time.
Article Snippet: Cells were transfected with pEGFPC1-human APPL1, a gift from Pietro De Camilli (Addgene plasmid #22198) , EGFP-APPL1-ΔPTB, a gift from Donna Webb (Addgene plasmid #59768) , DsRed-p150 217-548, a gift from Trina Schroer (Addgene plasmid #51146) ,
Techniques: Expressing, Standard Deviation